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| Sakamoto, Leo; Ichikawa, Yasuaki; Sakata, Yoshimi; Matsumoto, Kotaro; Inokuma, Hisashi; 松本, 高太郎; 猪熊, 壽. |
| The prevalence of Ehrlichia and Anaplasma in 1,427 dogs from 32 Japanese prefectures was evaluated by PCR and DNA nucleotide sequencing. PCR screening demonstrated that 18 dogs (1.3%) were positive for Anaplasmataceae. Sequence analysis revealed that 14 of the amplicons were most closely related to Wolbachia spp., symbionts of Dirofilaria immitis, whereas three were identified as Anaplasma bovis. The remaining amplicon could not be sequenced. Almost the entire sequence of 16S rRNA (1,452 bp) from one of the positive specimens was determined, and subsequent phylogenetic analysis confirmed that the detected sequence was that of A. bovis. This is the first detection of A. bovis DNA fragments in dogs. Species-specific nested PCR showed that 15 (1.1%) of the... |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3083 |
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